evaluate the frequency of foot and mouth disease viral carriers in slaughtered sheep in mashhad industrial abattoir using rt-pcr
نویسندگان
چکیده
background: foot -and- mouth disease (fmd) is endemic in iran. molecular techniques for diagnosis of persistent infection or carrier animals have shown a potential ability to improve the detection of a low genome copy number in samples. objectives: the purpose of this study is to evaluate the frequency of foot and mouth disease viral carriers in slaughtered sheep in mashhad industrial abattoir using rt-pcr. methods: samples were isolated from tonsil of 94 slaughtered sheep analyzed by rt-pcr experiment for the detection of fmd, identification of fmd virus serotypes and at the end nucleic acid sequencing were performed. results: the results showed that the 23 samples (24/5 percent) were positive for the presence of fmd virus rna, of which 89.9% of cases are type o and 3 cases of fmd samples did not respond. the results of the 1d genome sequencing of the nucleic acid virus showed that fmd virus of sheep (o/irn/100/2010sheep), has 92/02% similarity with the virus (o/irn/67/2001-2005) and 88/42% similarity with the virus (o/irn/15/2004-2008). conclusions: this study showed that the percentage of fmdv sheep carriers in mashhad slaughterhouse was remarkable. estimation of the frequency of carrier state in cattle and small ruminants is recommended as a monitor of control plan in the region.
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عنوان ژورنال:
تحقیقات دامپزشکیجلد ۷۰، شماره ۱، صفحات ۷-۱۴
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